kidney tissue sections  (R&D Systems)

Journal: Nature Communications

Article Title: Valence-engineered catalysis-selectivity regulation of molybdenum oxide nanozyme for acute kidney injury therapy and post-cure assessment

doi: 10.1038/s41467-024-53047-1

Figure Lengend Snippet: a Timeline of AKI modeling and treatment with mice. CRE ( b ) and BUN ( c ) levels in the blood serum from each group after indicated treatments ( n = 3; Data are presented as means ± SD. * P < 0.1, n.s. no significance. P values in ( b ): 2.0 × 10 – 2 , 2.1 × 10 – 2 , 1.8 × 10 – 1 and 2.3 × 10 – 2 , P values in ( c ): 3.4 × 10 – 2 , 2.9 × 10 – 2 , 2.6 × 10 – 1 and 5.6 × 10 – 2 ). d The survival rate of AKI mice treated with PBS and MF-0, respectively. e H&E staining of renal sections from each treatment group. Arrows indicated damaged tubules and asterisks indicated the formation of casts (a marker of more severe tubular damage). f DAPI (blue fluorescence indicating cell nuclei) and dihydroethidium (red fluorescence indicating ROS level) staining of kidney tissues from each treatment group. NAC used in ( b , c , e , f ) was a ROS inhibitor. The injection dosage of agents in different treatment groups was 200 μL: NAC (800 μg/mL), MF-0 (800 μg of Mo per mL). Significance was calculated by one-sided Student’s t -test. Source data are provided as a Source Data file.

Article Snippet: Photographs of cell imaging and kidney tissue section staining were obtained using a model eclipse Ti2-U inverted fluorescence microscope (Nikon).

Techniques: Staining, Marker, Fluorescence, Injection